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Biol Reprod 108(4):522537 Typiak M, Piwkowska A (2021) Antiinflammatory actions of klotho: implications for therapy of diabetic nephropathy

Due to the existence of the conjugated system of C = C bonds and carbonyl groups, 4-HNE can provide partial positive charge to the carbon at position three and become efficient electrophiles, allowing it to react with essential biological molecules, including proteins and DNA (107109)

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Thus, mouse hepatocytes used in this study have much strength and can be used for investigating the link between GSH-deficiency, oxidative stress, and VD regulatory genes